At least two weeks prior to sample submission for a 10x Genomics Chromium project:
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Meet with the Imaging Center staff to discuss your project. For optimal outcomes, it is best that both the researcher and the staff understand the requirements of the project and the technology. We can also help advise you in design and sample preparation.
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Submit a completed Project Safety Questionnaire. For the safety of the staff, it is necessary to understand the nature of any hazards that may be encountered with the cells to be processed. This should be submitted at least two weeks before initiating the project, as reagents will not be purchased until the Project Safety Questionnaire has been reviewed and approved.
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Schedule a sample submission appointment two weeks in advance. Reagents for the Chromium are not stocked, so must be ordered for each run. To allow time for order processing and delivery, and to set aside staff time for sample processing, we need about two weeks notice at this time.
- Consider a test run and evaluation of your sample prep. While not required, it is highly recommended that you schedule a trial run on your sample preparation in advance of bringing samples for 10x Chromium processing. We can evaluate your sample prep for viability, cell concentration, size, debris, and other factors that will influence the success of your run. Issues can then be identified and resolved before you bring your experimental samples for processing. There is no charge for this evaluation.
On the day of sample submission:
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Contact us when you are ready to come to the Imaging Center with your samples. If you have any changes to your schedule or if you must postpone a sample run, please contact us as soon as you know.
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Complete a Sample Submission Form and bring that with you to the Imaging Center with your samples. This may be provided either electronically or printed.
- Be prepared to stay until initial cell assessment is performed. Potential questions or problems may arise during evaluation of the submitted cells. These may include such issues as need to change cell concentration, resolve clumping of cells, or assess sub-optimal cell viability. It is necessary to be able to consult with the researcher quickly to resolve such issues and make determinations about the best course of action. Typically this evaluation will take about an hour, but depends on the number of samples.
